Skip to main content Accessibility help We use cookies to distinguish you from other users and to … Its complex life cycle and rich cell biology is reflected in more than 2500 publications on various aspects of its biochemistry, developmental biology, cytoskeleton, and cell motility. The life cycle of Physarum polycephalum as we … 1955 May 21; 175 (4464):890–891. The rate and extent of plasmodium formation were studied in mating tests involving pairs of largely isogenic amoeba1 strains compatible for matingtype ( mt ) alleles. Techniques were developed to facilitate genetic analysis of a Myxomycete, Physarum polycephalum.The uninucleate cells that form part of the life-cycle (the ‘amoebae’), in two-membered culture with Pseudomonas fluorescens, were found to be suitable for handling by some of the standard techniques of microbial genetics, including plating.. 2. The force generating system for these phenomena is cytoplasmic actomyosin. 1978 Jun 22; 519 (1):118–124. View Physarum Polycephalum Research Papers on Academia.edu for free. Using 50 percent growth inhibition of Physarum polycephalum (a coenocytic slime mold) as a screen for the selection of antitumor agents, 44 of the 195 compounds tested were effective at concentrations of <100 µg/ml. While the vast majority of spliceosomal introns are removed by the so-called major spliceosome, diverse eukaryotes also contain a mysterious second form, the minor spliceosome, and associated introns [[1][1]–[3][2]]. Morphological observations on growth and differentation of Physarum polycephalum grown in pure culture. 1961 May; 25:47–59. Eggehard Holler Institut für Biophysik und physikalische Biochemie der Universität Regensburg, D-93040 Regensburg, Germany * Corresponding author. 1985 Nov; 17 (4):229–238. We quantified the search pattern of plasmodia after engulfment of food of 6 different qualities. Physarum polycephalum is an amoeboid organism that forages as a flowing mass of pseudopods. [Google Scholar] SIRLIN JL. Proceedings of the Royal Society B: Biological Sciences, 278, 539 – 545. doi:10.1098/rspb .2010.1624 Google Scholar Frequency Coupling Model for Dynamics of Responses to Stimuli in Plasmodium of Physarum Polycephalum A Takamatsu, K Takahashi, M Nagao, Y Tsuchiya Journal of the Physical Society of Japan 66 (6), 1638-1646 , 1997 Random network peristalsis in Physarum polycephalum … Adamatzky, A.: Physarum Machines: Computers from Slime Mould. Abstract. A derivative line of the homothallic Colonia strain of Physarum polycephalum has been isolated which produces plasmodia with high efficiency within clones of amoebae. Intriguingly, such a spike of joint DNA molecules is often detectable in replicating DNA from mitotic cells. 2014. A systematic variability was observed: plasmodia formed either rapidly and extensively or slowly and inefficiently. P. polycephalum Responds to a Stimulus with a Propagating Change in Contraction Dynamics.. To follow the propagation of a stimulus throughout a P. polycephalum network we first observed networks before and after stimulation using bright-field microscopy over the course of 2 to 3 h. Data were analyzed to extract and track contractions along each tube (Materials and Methods). The cytoplasmic aspects of mitochondrial biogenesis have been the focus of much recent attention and a review is presented here of studies on the life cycle of mitochom dria inPhysarum polycephalum. [Europe PMC free article] [Google Scholar] DANIEL JW, RUSCH HP. Biochim Biophys Acta. Physarum polycephalum exhibits the same kind of economically irrational behaviour as has been described in humans (Latty and Beekman 2011a). Nature. Ltd., London (2010) Google Scholar Exp Cell Res. Physarum, or more precisely Physarum polycephalum, is an acellular slime mold, or myxogastrid. 1965 Apr; 38:33–41. Abstract. [Google Scholar] Evans TE. Chemical Science, 6 (5), 2859–2868. Spliceosomal introns interrupt nuclear genes and are removed from RNA transcripts (“spliced”) by machinery called spliceosomes. Google Scholar Physarum polycephalum: Yellow goo that thinks Physarum polycephalum is one of a group of strange species called "myxomycetes." W.M Wormington, R.F WeaverPhotoreceptor pigment that induces differentiation in the slime mold Physarum polycephalum Proc. 1. Food quality had a significant, positive effect on how long plasmodia waited before resuming search behavior and on how long it took to abandon food disks. Two-dimensional neutral/neutral agarose gel electrophoresis is used extensively to localize replication origins. Exp Cell Res 125: 441–452 CrossRef Google Scholar Jeffery WR, Rusch HP (1974) Induction of somatic fusion and heterokaryosis in two incompatible strains of Physarum polycephalum. Representation of shape mediated by environmental stimuli in Physarum polycephalum and a multi-agent model. Detailed knowledge of the life cycle is a prerequisite for meaningful genetic analysis of any organism. Google Scholar. The slime mould is efficient in imitating formation of man-made road networks, where major urban areas are sources of nutrients. Plasmodium of acellular slime mould Physarum polycephalum exhibits traits of wave-like behaviour. We used a similar approach to grow slime mould on a three-dimensional template of Moon to speculate on potential colonization scenarios. What is Physarum? The plasmodium’s behaviour can be finely tuned in laboratory experiments by using herbal tablets. Sci., U.S.A., 73 (1976), pp. Physarum polycephalum has been the subject of numerous studies as a living agent that could be integrated into a biological computer system in the future. Jacobson DN (1980) Locomotion of Physarum polycephalum amoebae is guided by a short–range interaction with E. coli. Shipley GL, Marrs J, Yang F. Growth and differentiation of wild type amoebae of Physarum polycephalum in liquid culture. CrossRef Google Scholar Physarum polycephalum, literally the 'many-headed' slime mold, is a giant multi–nucleated but unicellular protist.Since the time of its first description, it has been the subject of a multitude of cell biological, biochemical, genetic, and lately physical studies. This paper introduces a novel application of bionic engineering: a bionic musical instrument using Physarum polycephalum.Physarum polycephalum is a huge single cell with thousands of nuclei, which behaves like a giant amoeba. [Google Scholar] Affolter HU, Braun R. Ribosomal DNA in spores of Physarum polycephalum. Such studies have focused predominantly on behavior of the mitochondrial genome throughout the mitochondrial life cycle and have been designed to reveal details about (1) the role of … The strong similarities between disparate systems, representing different kingdoms of life, and different levels of biological organisation, suggest that collective biological systems may follow common design features. Principles of an object-oriented programming language for Physarum polycephalum computing. A hybrid living/organic electrochemical transistor based on the Physarum polycephalum cell endowed with both sensing and memristive properties. 1. It also detects X-shaped recombination intermediates in meiotic cells, in the form of a typical vertical spike. Radiation sensitivity and the mitotic cycle in Vicia faba. Using the synergistic effect of ultraviolet light and caffeine, mutants of this line have been isolated which fail to undergo the developmental transition between haploid amoebae and diploid plasmodia (apt mutants). It is known that this simple amoeboid organism has the ability to find the shortest route between two points in a labyrinth or on a ring (Nakagaki et al. In the paper, we present principles of an object-oriented programming language for Physarum polycephalum computing. Results of inhibition of KB cell culture screen are given for comparison. Dev Biol. The pure culture of Physarum polycephalum on a partially defined soluble medium. J Jones, R Mayne, A Adamatzky. Physarum polycephalum, an acellular plasmodial species belongs to the amoebozoa, a major branch in eukaryote evolution. Semantic Scholar uses AI to extract papers important to this topic. This method resolves DNA structures containing replication forks. J Gen Microbiol. During its foraging behavior this plasmodium produces electrical activity corresponding to different physiological states. 1961 Oct; 3:588–614. Abstract. Physarum polycephalum, literally the ‘many-headed’ slime mold, is a giant multi–nucleated but unicellular protist.Since the time of its first description, it has been the subject of a multitude of cell biological, biochemical, genetic, and lately physical studies. [Google Scholar] Tanaka K. Intranuclear microtubule organizing center in early prophase nuclei of the plasmodium of the slime mold, Physarum polycephalum. The slime mould exhibits some remarkable characteristics that have been exploited by researchers in a plethora of different research areas, including mapping (Tero et al., 2006, Adamatzky and Jones, 2010), robotic … [Google Scholar] McLEISH J. Semantic Scholar uses AI to extract papers important to this topic. The plasmodia of Physarum polycephalum show different oscillatory phenomena (time period approximately 1.3 min) in their contraction behaviour and their protoplasmic flow. Physarum polycephalum is a single celled, multi‐nucleate amoeboid organism that can attain sizes exceeding 930 cm 2 (Kessler 1982). 2016 Oscillations and uniaxial mechanochemical waves in a model of an active poroelastic medium: Application to deformation patterns in protoplasmic droplets of Physarum polycephalum [Google Scholar] MITTERMAYER C, BRAUN R, RUSCH HP. A gene conferring actidione resistance and abnormal morphology on Physarum polycephalum plasmodia - Volume 15 Issue 1 - Jennifer Dee, Russell T. M. Poulter. THE EFFECT OF ACTINOMYCIN D ON THE TIMING OF MITOSIS IN PHYSARUM POLYCEPHALUM. [Google Scholar] GUTTES E, GUTTES S, RUSCH HP. A single tablet acts as a fixed attractor: plasmodium propagates towards the tablet, envelops the tablet with its body and stays around the tablet for several days. Physarum polycephalum, because of its unique life cycle, is a useful organism in which to address a wide variety of questions of biological interest.These include problems relating to motility, differentiation, cell cycle regulation, and ultrastructural organization of the mitotic apparatus. Plasmodium formation was found to be 103- to 104-fold more extensive in "rapid" crosses than in "slow" crosses. Gröbner, P., and Loidl, 1984, Thymidine kinase: A novel affinity chromatography of the enzyme and its regulation by phosphorylation in Physarum polycephalum, J. Biol. A true slime mold, the plasmodium of Physarum polycephalum is an amoeboid organism with a sheet-like shape. 2014. Tel ... Physarum polycephalum, Aureobasidium sp. Biochem Biophys Res Commun. 3896-3899 CrossRef View Record in Scopus Google Scholar Acad. Myxogastrids are closely related to the cellular slime molds, or dictyostelids, including the well known Dictyostelium discoideum. Eggehard Holler. 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